Modification of The Method of Typing of The Human Apolipoprotein E Gene
DOI:
https://doi.org/10.61788/njn.v1i16.05Keywords:
apolipoprotein E, APOE, apolipoprotein E gene, apoE, rs429358, rs7412, alleles ε1, ε2, ε3, ε4, isoforms E2, E3, E4Abstract
A modification of the method of typing of human apolipoprotein E gene described by Hixson JE and Vernier DT. The analysis of the complete sequence of the apolipoprotein E (NG 007 084) and the nucleotide sequences of SNP rs429358 and rs7412 identified six nucleotide mismatches in the structure of the forward primer and reverse seven. Despite the impossibility of carrying out PCR amplification using oligonucleotide primers described Hixson JE and Vernier DT method was repeated several times with the publication of the results of several authors. To resolve this discrepancy was designed structure of oligonucleotide primers which give specific amplification products and carry out genotyping using endonuclease Hha I.
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